Karakteristik dan Peran Protein Ikat Folat (PIF)

Subandrate Subandrate, Dwirini Retno Gunarti, Mohamad Sadikin

Abstract


Asam folat merupakan kelompok vitamin B. Asam folat terikat dengan suatu protein yang disebut protein ikat folat (PIF). PIF merupakan suatu glikoprotein dengan 222 residu asam amino dan berat molekul sekitar 31000 Dalton. Struktur tiga dimensi PIF dipertahankan oleh enam sampai delapan jembatan disulfida. PIF memiliki kemampuan mengikat asam folat dengan perbandingan ikatan 1:1 mol. Situs aktif PIF mengandung gugus imidazol atau gugus sulfhidril. Kofaktor ion kalsium tidak dibutuhkan PIF dalam mengikat asam folat. Di dalam tubuh, PIF berperan menghimpun, mengangkut, dan membantu penyimpanan, penggunaan serta pendistrubusian asam folat ke jaringan. PIF juga berperan mengurangi ekskresi asam folat di ginjal dan meningkatkan penyerapan asam folat di intestinum. Dalam bidang kedokteran PIF dapat dimanfaatkan untuk mengukur kadar asam folat serum dengan metode analog ELISA.


Full Text:

PDF

References


Murray RK, Grannu DK, Mayes PA, Rodwell VW (editor). Harper’s illustrated biochemistry. Edisi ke-27. New York: McGraw-Hill. 2006. p516-7

Nelson DL, Cox MM. Lehninger principles of biochemistry. Edisi keempat. p672-83. Diunduh dari: www.whfreeman.com/lehninger4e.com

Koolman J, Roehm KH. Color atlas of biochemistry. Edisi kedua. Thieme Stuttgart: New York; 2005. p366

Svendsen IB, Martin B, Pedersen TG, Hansen SI, Holm J, Lyngbye J. Isolation and characterization of the folate-binding protein from cow's milk. Carlsberg Res. Commun. 1979; 44: 89-99

Verwei M, Arkbåge K, Mocking H, Havenaar R, Gr J. The binding of folic acid and 5-methyltetrahydrofolate to folate-binding proteins during gastric passage differs in a dynamic in vitro gastrointestinal model. J. Nutr. 2004; 134: 31–7

Babol NL. Folate binding protein in bovine milk: occurrence and properties studied with surface plasmon resonance. Tesis Doktor. Uppsala: Swedish University of Agricultural Sciences. 2007

Kim YI. Will mandatory folic acid fortification prevent or promote cancer? Am J Clin Nutr. 2004; 80: 1123– 8.

Sralsjo L, Arkbage K, Witthoft C, Jagerstad M. Evaluation of a radioprotein binding assay (RPBA) for folate analysis in berries and milk. Food Chemistry. 2002; 709:525-34

Sadikin M, Mardiana, Harahap IP. Folic acid technique by protein-ligans binding methode using folate binding protein from cow’s milks as binder protein. Dipresentasikan pada The 4th Indonesian Biotechnology Conference. Bogor. 2008

Mardiana. Penggunaan protein ikat folat dari susu sapi untuk mengukur kadar folat serum. Tesis Magister Biomedik. Jakarta: Universitas Indonesia; 2005

Nygren L, Sternesjo A, Bjorck L. Determination of folate-binding proteins from milk by optical biosensor analysis. International Dairy Journal. 2003; 13: 283–90

Nixon P, Jones M, Winzor DJ. Quantitative Description of the interaction between folate and the folate-binding protein from cow’s milk. Biochem. J. 2004; 382: 215–21

Holm J, Hansen SI. Ligand binding and polymerization characteristics of human milk folate binding protein depend on concentration of purified protein and presence of amphiphatic substances. Bioscience Reports. 2003. 23(2&3)

Holm J, Hansen SI. Binding of radiolabeled folate and 5-methyltetrahydrofolate to cow’s milk folate binding protein at ph 7.4 and 5.0. relationship to concentration and polymerization equilibrium of the purified protein. bioscience reports. 2001; 21(6)

Bailey LB (editor). Folate in health and disease. Edisi kedua. Boca Roton: CRC Press; 2010

Sigma-Aldrich. Folic acid metabolism: a role in cancer's cause and cure. Biofiles. 5 (6): 24-9.

Arkbåge K. Vitamin B12, folate and folate-binding proteins in dairy products: analysis, process retention and bioavailability. Tesis Doktor. Uppsala: Swedish University of Agricultural Sciences. 2003

Brody T. Nutritional biochemistry. Edisi kedua. California: Academic Press. 1998. p493-516

Gregory III JF. Chemical and nutritional aspects of folate research: analytical procedures, methods of folate synthesis, stability, and bioavaillability of dietary folates. Advances in food and nutrition research. 1989; 33

Shane B. Folate fortification: enough already? Am J Clin Nutr. 2003;77:8–9

Sauer J, Mason JB, Choi SW. Too much folate – a risk factor for cancer and cardiovascular disease? Curr Opin Clin Nutr Metab Care. Januari 2009; 12(1): 30-6

Sweeney MR, McPartlin J, Scott J. Folic acid fortification and public health: report on threshold doses above which unmetabolised folic acid appear in serum. BMC Public Health. 2007; 7(41): 1-7

Purwani E, Zulaekah S. Risiko lahirnya bayi cacat pembuluh syaraf pada ibu hamil yang kekurangan asam folat. Jurnal Kesehatan. Juni 2008; 1(1):19-28

Albert CM, Cook NR, Gaziano JM, Zaharris E, MacFadyen J, Danielson E et al. Effect of folic acid and b-vitamins on risk of cardiovascular events and total mortality among women at high risk for cardiovascular disease: a randomized trial. JAMA. 7 Mei 2008; 299(17): 2027–36

Spiegelstein O, Cabrera RM, Bozinov D,Wlodarczyk B, Finnell RH. Folate-regulated changes in gene expression in the anterior neural tube of folate binding protein-1(folbp1)-deficient murine embryos. Neurochemical Research. Juni 2004; 6(29): 1105-12

Ashraf MJ, Cook JR, Rothberg MB. Clinical utility of folic acid testing for patients with anemia or dementia. J Gen Intern Med. 2008; 23(6):824–6

Henne WA, Doorneweerd DD, Lee J, Low PS, Savran C. Detection of folate binding protein with enhanced sensitivity using a functionalized quartz crystal microbalance sensor. Analytical Chemistry. 15 Juli 2006; 78: 4880-4

Holm J, Hansen SI. Characterization of a high affinity folate binding protein in porcine serum: ionic charge, concentration—dependent polymerization and ligand binding mechanism. Bioscience Reports. Oktober & Desember 2003; 23 (5&6).

Salter DN, Scott KJ, Slade H, Andrews P. The preparation and properties of folate-binding protein from cow's milk. Biochem. J. 1981; 193:469-76

Svendsen IB, Hansen SI, Holm J, Lyngbye J. The complete amino acid sequence of the folate-binding protein from cow's milk. Carlsberg Res. Commun. 1984; 49: 123-31

Holm J, Hansen SI, Hoier-Madsen M. ionic charge, hydrophobicity and tryptophan fluorescence of the folate binding protein isolated from cow’s milk. Bioscience Reports. Juni 2001; 21(3).

Holm J, Hansen SI. Effect of hydrogen ion concentration and buffer composition on ligand binding characteristics and polymerization of cow’s milk folate binding protein. Bioscience Reports. Desember 2001; 21(6)

Subandrate, Gunarti DR, Sadikin M. Properties of folate binding protein purified from cow’s milk. HAYATI Journal of Biosciences. 2012;19(3): 105-109

Maziarz KM, Monaco Hl, Shen F, Ratnam M. complete mapping of divergent amino acids responsible for differential ligand binding of folate receptors α and β. Journal of Biological Chemistry. April 1999; 274(16): 11086–91

Holm J, Hansen SI, Hoier-Madsen M, Bostad L. A high-affinity folate binding protein in proximal tubule cells of human kidney. Kidney International. 1992; 41: 50-5

Verwei M, Berg HVD, Havenaar R, Groten JP. Effect of folate-binding protein on intestinal transport of folic acid and 5-methyltetrahydrofolate across caco-2 cells. Eur J Nutr. 2005; 44: 242–9


Refbacks

  • There are currently no refbacks.


ISSN (Print) : 2406-7431
E-ISSN (Online) : 2614-0411
 
Editorial Office
Fakultas Kedokteran Universitas Sriwijaya
Jl. Dr. Moehammad Ali Kompleks RSMH Palembang 30126, Indonesia
Telp. 0711-352342, Fax.: 0711-373438
email: jurnalfkunsri@gmail.com

 
Creative Commons License
Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya by https://ejournal.unsri.ac.id/index.php/jkk is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.