HYDROLYSIS OF AMYLASE INHIBITORS FOR CASSAVA (Manihot esculenta crantz) ADIRA-1 VARIETY

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Hydrolysis performed onamylase inhibitorsthat had the highest inhibitory propertie sinprevious studi esamylase inhibitors on sample No.2 (P1L2). Hydrolysis test performed at several volume amylase inhibitors. Amylase enzyme used for hydrolysis test derived from Brevibacteriumsp with various volumes. Amylase inhibitor volume by ammonium sulphate results in some of these volumes was reacted with the enzymeamylase from Brevibacteriumspatvarying temperatures for 10 minutes using sodium phosphate buffers of varying pH, and then added to the reaction was added a few mL of starch (Merck) with various concentrations. For 100% inhibition of activity of amylase inhibitors on the mechanism of action was achieved at a volume of amylase inhibitors of  400 ng, , amylase volume of 0.4 mL, a temperature of 30° C, pH 6.7 and 1% starch concentration.