Effect of Jati Belanda Leaves Extract on Myeloperoxidase Level in Wistar Rat's Lung Induced Contusion Pulmonum

Nia Savitri Tamzil, Evi Lusiana, Desi Oktariana

Abstract


Pulmonary contusions are injuries to the lung parenchyma that often result from blunt trauma to the chest wall. This injury will activate the inflammatory response which can produce the effects of oxidative stress so that eventually lung damage occurs. Several studies have identified the effects of Jati Belanda leaves extracts related to the inflammatory process and their effects as antioxidants. This research is an in vivo experimental study with a prepost-test with control group design approach that aims to determine the effectiveness of the extracts of Jati Belanda (Guazuma ulmifolia) in its protection against pulmonary alveolar cells by pretreatment pulmonary contusions. The subjects of this study were white rats (Rattus norvegicus) Wistar strain which were divided into 5 groups namely positive control, negative control, Dutch teak leaf extract 125 mg / kgBB, 250 mg / kgBB and 500 mg / kgBW. Rats were induced by dropping a weight of 400 grams as high as 50 cm. The results of the study of Jati Belanda Leaf Extract (EDJB) all doses and positive control can significantly reduce levels of myeloperoxidase expression (p <0.05). Therefore, it can be concluded that the extracts of Jati Belanda leaves (Guazuma ulmifolia) are all effective doses in reducing the expression of myeloperoxidase in pulmonary tissue of Wistar rats induced by pulmonary contusions.


Keywords


pumonary contusion, jati belanda, myeloperoxidase, in vivo

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References


Miller PR, Croce MA, Bee TK, Qaisi WG, Smith CP, Collins GL, Fabian TC. 2001. ARDS after pulmonary contusion: Accurate measurement of contusion volume identifies high risk patient. J Trauma. 51: 223-228

Tarladacalisir YT, Tarladacalisir T, Sapmaz-Metim M, Karamustafaoglu A, Uz YH, Akpolat M, Cerkezkayabekir A, Turan FN. 2014. N-acetylcysteine counteracts oxidative stress and protects alveolar epithelial cells from lung contusion- induced apoptosis in rats with blunt chest trauma. J Mol Hist.

Raghavendran K, Notter RH, Davidson BA, Helsinki JD, Kunkel SL, Knight PR. 2009. Lung Contusion: Inflammatory Mechanisms and Interaction with Other Injuries. Shock. 32(2): 122-130

Ayan E, Koksel O, Polat A, Tamer L, Ersoz G, Demir M, et al. The role of thoracic trauma in inflammatory responses, apoptosis and bacterial translocation following multiple traumas. Ulus Travma Acil Cerr Derg 2013; 19(6):491-9

Laudy S, Donabauer B, Busch T, Kerner T, Bercker S, Bail H, et al. Low incidence of multiple organ failure after major trauma. Injury. 2007;38:1052-8

Lazarus HM, Fox J, Burke JP, Lloyd JF, Snow GL, Mehta RR, et al. Trauma patient hospital-associated infections: risks and outcomes. J Trauma. 2005;59:188-94

Sies H. 1991. Oxidative Stress: From Basic Research to Clinical Application. Am J Med. 91(3C):31S-38S.

Hawkey CJ. 2000. Non steroidal anti-inflammatory drug gastropathy. Gastroenterology 119, 521-35.

Liener U, Knorfel M, Strater J, Barth T. 2003. Induction of apoptosis following blunt chest trauma. Shock. 20: 511-6

Sutyak JP, Wohltmann CD, Larson J. Pulmonary contusions and critical care management in thoracic trauma. Thorac Surg Clin 2007;17(1):11-23

Moloney JT, Fowler SJ, Chang W. Anesthethic management of thoracic trauma. Curr Opin Anaesthesiol. 2008;21(1):41-6

Lucid WA, Taylor TB, Strang GR. Pediatric Emergency Medicine: A Comprehensive study guide. New York: McGraw-Hill:2002. Thoracic trauma; pp.92-100

Caballero-George C, Vanderheyden PM, Solis PN, Pieters L, Shaha AA, Gupta MP, Vauquelin G, Vlietinck AJ. 2001. Biological screening of selected medicinal Panamian plants by radioligand-binding-techniques. Phytomedicine 8:59-70.

Tombolangi AK. 2004. Khasiat ekstrak daun jati belanda (Guazuma ulmifolia Lamk) terhadap kadar lipid peroksidase darah kelinci yang hiperlipidemia (Skripsi). Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam; Institut Pertanian Bogor.

Andriani Y. 2004. Ekstrak daun jati belanda (Guazuma ulmifolia Lamk) mencegah hiperlipidemia dan perkembangan aterosklerosis pada kelinci (tesis). Bogor: Sekolah Pascasarjana: Institur Pertanian Bogor

Gonzales-Gomez JC, Ayala-Burgos A, Gutierrez-Vazquez E. 2006. Total Phenols and condensed tannins in tree species with potential as forage sources in the tropics. Livestock Research for Rural Development.

Berenguer B, Trabadela S, Sanchez-Fidalgo A, Quilez, P.Mino, De La Puerta R, Martin-Calero MJ. 2007. The Aerial parts of Guazuma ulmifolia Lamk protect against NSAID-induced gastric lession. J Ethno. 114: 153-160

Delporte C, Backhouse N, Erazo S, Negrete R, Vidal P, Silva X, Lopez-Perez JL, Feliciano AS, Munoz O. 2005. Analgesic-antiinflammatory properties of Proustia pyrifolia. J Ethno. 99: 119-124




DOI: https://doi.org/10.32539/bji.v5i3.10009

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