THE BIOMOLECULAR CHARACTERISTICS OF ANGIOTENSIN II TYPE 1 RECEPTORS AS PARAMETERS IN KIDNEY FIBROSIS: A BIOINFORMATICS ANALYSIS

Evi Lusiana, Irfannuddin Irfannuddin

Abstract


AGTR1 is a modulator of angiotensin II-induced signal transduction. This article aims to examine the biomolecular characteristics of AGTR1 and the role of AGTR1 in the renal fibrosis pathway in Rattus norvegicus rats. The gene ID NC 051352.1 and protein ID NP 112271.2 for AGTR1 were retrieved from the National Center for Biotechnology Information's website. The 'NCBI' website has details on AGTR1's structure, location, and expression. Protease prediction using 'PEPTIDE CUTTER'; glycosylation index prediction using NETNGLYC; and target protein location evaluated on the 'TARGETP' homepage. AGTR1 in Rattus norvegicus is located on chromosome 17p12, This protein has a stability score of 34.48. The aliphatic index was 114.62. AGTR1 is found inside, outside, and on the transmembrane, as determined by the THMM. Only 21 enzymes were predicted to be capable of cleaving the AGTR1 protein, out of a total of 37 protease. AGTR1 protein has four glycosylated amino acid sequences. AGTR1 protein is primarily at other location 0.850, secretory pathway (0.590), and a little amount in mitochondria (0.018AGTR1/AT1R is a marker for renal fibrosis in the renin angiotensin system, which can lead in vasoconstriction, inflammation, fibrosis, sodium retention, and water retention. The AGTR1 protein's molecular feature is a determinant in the progression of renal fibrosis, revealed to a bioinformatics study.


Keywords


AGTR1, AT1R, Bioinformatic, Biomolecular

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Nogueira A, Pires MJ, Oliveira PA. Pathophysiological mechanisms of renal fibrosis: a review of animal models and therapeutic strategies. In Vivo (Brooklyn) [Internet]. 2017; Available from: https://iv.iiarjournals.org/content/31/1/1.short

Panizo S, Martínez-Arias L, Alonso-Montes C, Cannata P, Martín-Carro B, Fernández-Martín JL, et al. Fibrosis in chronic kidney disease: Pathogenesis and consequences. Vol. 22, International Journal of Molecular Sciences. 2021.

Mezzano SA, Ruiz-Ortega M, Egido J. Angiotensin II and renal fibrosis. Vol. 38, Hypertension. 2001.

Fountain JH, Lappin SL. Physiology, renin angiotensin system [Internet]. europepmc.org; 2017. Available from: https://europepmc.org/article/nbk/nbk470410

Matavelli LC, Siragy HM. AT2 receptor activities and pathophysiological implications [Internet]. Journal of cardiovascular pharmacology. ncbi.nlm.nih.gov; 2015. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355033/

Nishida M, Fujinaka H, Matsusaka T, Price J, Kon V, Fogo AB, et al. Absence of angiotensin II type 1 receptor in bone marrow-derived cells is detrimental in the evolution of renal fibrosis. J Clin Invest. 2002;110(12).

Aoyagi Y, Furuyama T, Inoue K, Matsuda D, Matsubara Y, Okahara A, et al. Attenuation of Angiotensin II–Induced Hypertension in BubR1 Low-Expression Mice Via Repression of Angiotensin II Receptor 1 Overexpression. J Am Heart Assoc. 2019;8(23).

Biotechnology NC for, Information. Agtr1a angiotensin II receptor, type 1a [Rattus norvegicus (Norway rat)] - Gene - NCBI [Internet]. www.ncbi.nlm.nih.gov. [cited 2022 Apr 18]. Available from: https://www.ncbi.nlm.nih.gov/gene/24180

www.rgd.mcw.edu. Agtr1a (angiotensin II receptor, type 1a) - Rat Genome Database [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://rgd.mcw.edu/rgdweb/report/gene/main.html?id=2070#expression

www.uniprot.org. Agtr1 - Type-1A angiotensin II receptor - Rattus norvegicus (Rat) - Agtr1 gene & protein [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://www.uniprot.org/uniprot/P25095

www.ncbi.nlm.nih.gov. type-1B angiotensin II receptor (Rattus norvegicus) [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://www.ncbi.nlm.nih.gov/ipg/NP_112271.2

Oro C, Qian H, Thomas WG. Type 1 angiotensin receptor pharmacology: Signaling beyond G proteins. Vol. 113, Pharmacology and Therapeutics. 2007.

Zhang H, Unal H, Gati C, Han GW, Liu W, Zatsepin NA, et al. Structure of the angiotensin receptor revealed by serial femtosecond crystallography [Internet]. Cell. Elsevier; 2015. Available from: https://www.sciencedirect.com/science/article/pii/S0092867415004286

Protparam. ExPASy ProtParam tool [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://web.expasy.org/cgi-bin/protparam/protparam

Protscale. ProtScale analysis [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://web.expasy.org/cgi-bin/protscale/protscale.pl?1

TMHMM. TMHMM - 2.0 - Services - DTU Health Tech [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://services.healthtech.dtu.dk/service.php?TMHMM-2.0

Lopez-Ilasaca M, Liu X, Tamura K, Dzau VJ. The Angiotensin II Type I Receptor-associated Protein, ATRAP, Is a Transmembrane Protein and a Modulator of Angiotensin II Signaling. Mol Biol Cell. 2003;14(12).

PeptideCutter. ExPASy - PeptideCutter [Internet]. 2020 [cited 2022 Apr 26]. Available from: https://web.expasy.org/cgi-bin/peptide_cutter/peptidecutter.pl

NetNGlyc. NetNGlyc - 1.0 - Services - DTU Health Tech [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://services.healthtech.dtu.dk/service.php?NetNGlyc-1.0

TargetP. TargetP - 1.1 - Services - DTU Health Tech [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://services.healthtech.dtu.dk/service.php?TargetP-1.1

SWISS-MODEL. Protein AGTR1 Structure [Internet]. 2020 [cited 2022 Apr 10]. Available from: https://swissmodel.expasy.org/interactive/S2Ly7r/templates/

Guo DF, Sun YL, Hamet P, Inagami T. The angiotensin II type 1 receptor and receptor-associated proteins. Vol. 11, Cell Research. 2001.

KEGG PATHWAY. KEGG PATHWAY: Renin-angiotensin system - Reference pathway [Internet]. 2021 [cited 2022 Apr 10]. Available from: https://www.kegg.jp/kegg-bin/highlight_pathway?scale=1.0&map=map04614&keyword=angiotensin II receptor

Eguchi S, Kawai T, Scalia R, Rizzo V. Understanding Angiotensin II type 1 receptor signaling in vascular pathophysiology. Hypertension. 2018;71(5).

Eshraghian A, Iravani S, ... The association between angiotensin II type 1 receptor gene A1166C polymorphism and non-alcoholic fatty liver disease and its severity [Internet]. Middle East Journal of …. ncbi.nlm.nih.gov; 2018. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040929/




DOI: https://doi.org/10.32539/mks.v54i2.17535

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